• End of Registration
  • jun 15 2021
  • Classes Start
  • apr 20 2021
  • Classes End
  • jun 15 2021
  • Estimated Effort
  • 2 h/week
  • Language
  • French

About the course

Microscopy technologies have progressed to such a point that it is now possible to image a developing vertebrate at the single cell resolution. Two main model species are being used for such studies, the zebrafish and the mouse.

The present MOOC introduces the trainees to these two animal models, to the most modern microscopy technologies, to the use of high volume of data (images) and to the modelling of the studied processes. Trainees will also be introduced to the intravital study of viral infections.

The MOOC gives both a general introduction and introduces to more specialized tools and concepts.

At the end of the MOOC, you will know:

  • How to get vertebrate embryos to be imaged
  • How to label embryos and larvae for imaging
  • How to choose the right microscope for your purpose
  • How to deal with both the type of data generated and with the large amount of images/data generated by your microscope
  • How to choose the right modeling tools and what modelling could bring to the experimental plans and to the understanding of the underlying biological processes.

Course format

The MOOC is made of 5 weeks of electronic lessons, and you will be offered quizzes to evaluate your performances

Prerequisites

The target audience are :

  • Students in their first years at the University, before graduating a Bachelor, in order for them to be introduced to the challenges of intravital imaging in vertebrates before their first laboratory internship.
  • Master students interested in these disciplines or graduated students and researchers that want to implement research projects in these disciplines.

Prerequisite : Basic knowledge in biology

The MOOC team

Tamara Sipka

from Serbia, Marie Skłodowska-Curie PhD student at the University of Montpellier, France

Salome Munoz

from Chile, Marie Skłodowska-Curie PhD student at Leiden University, The Netherlands

Radoslaw Gora

from Poland, Marie Skłodowska-Curie PhD student at Leiden University, The Netherlands

Hanh Nguyen

from Vietnam, Marie Skłodowska-Curie PhD student at CNRS, Paris, France

Mageshi Kamaraj

from India, Marie Skłodowska-Curie PhD student at CNRS, Paris, France

Antonia Weberling

from Germany, Marie Skłodowska-Curie PhD student in Cambridge, UK

Markjoe Uba

from Nigeria, Marie Skłodowska-Curie PhD student at STUBA, Slovakia

Seol Ah Park

from Corea, Marie Skłodowska-Curie PhD student at STUBA, Slovakia

Matteo Bernardello

from Italy, Marie Skłodowska-Curie PhD student at ICFO, Barcelona, Spain

Joel Dokmegang

from Cameroon, Marie Skłodowska-Curie PhD student at Manchester Metropolitan University, UK

Laurent Thomas

from France, Marie Skłodowska-Curie PhD student at Acquifer, Heidelberg, Germany

Polycarp Okock

from Kenya, Marie Skłodowska-Curie PhD student at TatraMed, Bratislava, Slovakia

Svetlana Jovanic

from Serbia, Marie Skłodowska-Curie PhD student at CNRS, Paris, France

Valerio Laghi

from Italy, Marie Skłodowska-Curie PhD student at Institut Pasteur, Paris, France

Course outline

  • Week 1 : Preparing embryos for imaging
  • Week 2 : What to image; Labelling techniques
  • Week 3 : How to image, Microscopes
  • Week 4 : From images to data
  • Week 5 : Modelling

Evaluation method

At the end of each week, quizzes are available to evaluate your acquired knowledge.

Partners

Terms of use

TERMS OF USE FOR COURSE CONTENT

Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

NonCommercial — You may not use the material for commercial purposes.

ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

CONDITIONS FOR THE USE OF CONTENT PRODUCED BY LEARNERS

Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

NonCommercial — You may not use the material for commercial purposes.

ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.